Putative nanobacteria represent physiological remnants and culture by-products of normal calcium homeostasis.
Putative living entities called nanobacteria (NB) are unusual for their small sizes (50-500 nm), pleomorphic nature, and accumulation of hydroxyapatite (HAP), and have been implicated in numerous diseases involving extraskeletal calcification.By adding precipitating ions to cell culture medium containing serum, mineral nanoparticles are generated t